The plate is washed to absorb non-specific binding, that is, it does not react with your bag on the board. I want you to have a residue in the drink. You have to wash it several times to wash off the inside. And what really wraps on your board, such as primary anti-secondary antibody, will be very tightly combined and can't be washed away. If you don't wash it, those residuals will affect your results, produce false positives, and optimize one of the focus of ELISA background. In the wash, the washing step can reduce the background signal caused by the unbound antibody, and how to increase the signal to noise ratio of the analysis.
There are two methods for washing the board:
1. Automatic: If there is an automatic washing machine, it should be used in the formal experiment process after skilled use.
2. Hand: suck or remove the liquid in the microplate, here Shanghai Hengyuan should remind friends to remember not to touch the slab; put several layers of absorbent paper on the test bench, and take a few times with the microplate; The recommended wash buffer is injected into the well at least 0.3 ml, soaked for 1-2 minutes, and the process is repeated several times as needed.
use
1. The amplification of the enzyme-catalyzed reaction is combined with the high specificity and specificity of the antigen-antibody affinity reaction, and the enzyme-labeled antigen or antibody is used as a main reagent for immunoassay, so that it has high sensitivity.
2. Realize the location of the antigen or antibody at the cellular or subcellular level, or quantify it at micrograms or even nanograms.
3. Its antigen-antibody reaction is highly specific. 
The production process of a machine is the whole process of making a product from a raw material (or semi-finished product). For machine production, it includes the transportation and preservation of raw materials, preparation for production, the manufacture of blank, the processing and heat treatment of parts, the assembly and debugging of products, paint and packaging. The content of production process is very extensive. Modern enterprises use the principles and methods of systems engineering to organize production and guide production, and regard the production process as a production system with input and output.
The technological process
The technological process
In the production process, the process of changing the shape, size, position and nature of the production object, so that it becomes a finished product or semi-finished product is called the technological process. It is an essential part of the production process. Process: casting, forging, stamping, welding, machining, assembly processes, such as machinery manufacturing process generally refers to the part machining process and machine of the sum of the assembly process, other process is known as the auxiliary process, such as transportation, storage, power supply, equipment maintenance, etc. The technological process is composed of one or several sequential processes, and a process is composed of several working steps.
Working procedure is the basic unit of mechanical processing process. The so-called process refers to a (or a group of) workers, in a machine tool (or a working place), on the same workpiece (or at the same time to several workpiece) of the continuous completion of that part of the process. The main characteristic of a process is that it does not change the processing object, equipment and operator, and the content of the process is continuously completed.
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