Elisa kit small experience sharing: how to do improper sample preservation - Database & Sql Blog Articles

I often encounter many students asking us to order the kits, saying that their samples are not properly stored, what should I do? Today we will explain this to you:

Specimens are stored improperly in the refrigerator for long-term specimens. IgG in serum can be polymerized into multimers, AFP can form dimers, which can lead to excessive background and even false positives in indirect ELISA; specimen placement time If it is too long (such as more than one day), sometimes the antigen or antibody immunological activity is weakened, and false negatives may also occur. In order to overcome the above interference, the serum samples determined by ELISA should be freshly collected; if it cannot be measured immediately, the serum samples measured within 5 days can be stored at 4 °C, and the serum samples measured after 1 week should be cryopreserved; the specimens melted after cryopreservation The protein is partially concentrated and unevenly distributed. It should be mixed after thorough measurement, but it should be gentle when mixing, and should not be strongly oscillated.

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Shanghai Xinfan Biological Company's main agent products:

Elisa kit: imported ELISA kit, domestic ELISA kit, human elisa kit, rat ELISA kit, mouse ELISA kit and so on.
Medium: microbial culture medium, chromogenic medium, BD medium, gibco medium, and the like.
Serum: fetal bovine serum, human serum, animal serum, NQBB, Hyclone, Gbico original serum.
Biological reagents: Amresco, Sigma, ABM, BIO-RAD, BD, ABCAM and other imported reagents.
Standard reference substance: imported reference substance, imported reference medicine, imported standard.
Antibodies: primary antibody, secondary antibody, flow antibody, etc.
Radio-disabled kit: imported radio-removal kit, domestic radio-removal kit, imported American Phoenix and other well-known brands.
Laboratory test services: radioimmunoassay, WB experiment, immunohistochemistry, fluorescence quantitative PCR, pathological cell staining, biochemical test.