Shanghai Yuanmu detailed: ELISA kit experimental preparation 6 elements! -Huaqiang Electronic Network

Shanghai Yuanmu detailed: elisa kit experimental preparation 6 elements!

The preparation before the ELISA experiment has a key factor in the success or failure of the experiment. The wrong step can affect the overall experimental results, so do you know the 6 elements of the experimental preparation? Follow Shanghai Yuanmu to see what time it is. Do the following and your experiment has been half successful.

1. Remove the kit from the refrigerator 1 hour before the experiment, and return the various reagents to room temperature to make the results more stable.

2. During the experiment, keep the substrate in the dark.

3. The speed of sucking the liquid with a gun should not be too fast, so as to avoid air bubbles and make the suction amount inaccurate.

4. When sucking the liquid, it is necessary to remove the gun with a dose and a quantity close to the required amount to reduce the error.

5. To ensure the accuracy of the pipette, the error should not exceed 2%. It can be determined with water and an electronic balance. But it is best to have a professional to correct it.

6. It is necessary to equip each of 20ul, 50ul, 100ul, 1000ul and a row of guns. After picking up different liquids, replace the tip. Even when taking standards.

The ELISA kit supplied by our company is one of the most widely used technologies in protein detection technology because of its simple operation and high sensitivity. Our ELISA kits are available in human, rat, mouse, cow, sheep, horse, rabbit, chicken, fish, duck, etc. You can also call the required kit!