Professor Yang Chaoyong of Xiamen University has recently successfully developed a new instrument method based on digital microfluidics and surface-enhanced Raman ultra-sensitive automated immunoassay, and related research results "Highly Sensitive and Automated Surface Enhanced Raman Scattering-based Immunoassay for H5N1 Detection "With Digital Microfluidics" published in "Analytical Chemistry" (Anal. Chem., 2018, DOI: 10.1021/acs.analchem.8b00002).
Traditional microfluidic technology greatly reduces the consumption of samples and reagents, and greatly increases the speed of analysis, but the bottleneck is that it relies on complex devices such as microchannels, microvalves, and micropumps, and the limitations of the manufacturing process are great. Technical staff requirements are relatively high. In recent years, the emerging digital microfluidics (DMF) has attracted wide attention in various fields. It is a novel droplet based on the dielectric wetting effect to realize precise and automated manipulation of discrete droplets on microelectrode arrays. Manipulation technology. The DMF technology uses “electricity†to drive the droplets, which realizes the operations of generating, moving, mixing and splitting the nanoliter to micro-upgraded droplets. It has the characteristics of low cost, easy processing, easy integration, high parallelism and full automation. It is of great significance for sample preparation, reaction, separation and detection.
Based on the advantages of precise control and automation of discrete droplets, the team conducted ultra-sensitive and automated immunodetection on the digital microfluidic platform. The pre-prepared samples and reagents are respectively introduced into the “pool†of the chip, and the software controls the “drop†of the sub-drops from the reservoir and the repeated incubation with the magnetic beads coated with the captured antibody. Cleaning and other steps. The surface-enhanced Raman probe was used to amplify the signal of the antigen to achieve a rapid, sensitive and automated detection of the influenza virus H5N1, and was successfully used for highly sensitive detection of serum complex samples.
The automation of digital microfluidics greatly simplifies the detection procedure, shortens the detection time, and reduces the risk of infectious samples detecting infections; at the same time, the reduction of sample and reagent consumption greatly reduces the detection cost; and, the precise control of discrete droplets The reconfigurability of the path makes the digital microfluidic chip a powerful droplet manipulation platform promising for more biological research and analysis.
Digital microfluidics is the new direction of cooperation between Yang Chaoyong's research group and Lin Shuichao's teacher in the past two years. In addition to the above applications of automated immunoassays, the team also successfully developed handheld DNA sequencers, automated single-cell analysis, and automated biological evolution characterization, and achieved breakthroughs by combining electrical, optical, and Raman detection methods. Sexual results.
Under the guidance of Prof. Yang Chaoyong, the research work was mainly completed by Wang Yang, a master of chemical engineering of 2016 grade, and Zhao Qingyu (co-first author) of 2015 iChEM Zhibo. This work was supported and supported by the National Natural Science Foundation of China (project approval number, 21435004, 21775128, 21705024, 21521004), Changjiang Scholars and innovation team development plan innovation team (approval number: IRT13036).
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