Determination of Vitamin B4 by UV-1100 Ultraviolet Spectrophotometry - Master's thesis

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Determination of Vitamin B4 by UV-1100 Ultraviolet Spectrophotometry

Key words: ultraviolet spectrophotometry; vitamin B4; US equipment ; UV-1100 vitamin B4 raw materials and tablets are contained in the Ministry of Health drug standards, content determination has been using Kjeldahl method, The method is cumbersome and time consuming. Vitamins are now absorbed in the ultraviolet region and have been used for the identification of drug substances, but no reports have been published for determining tablet content. We have tried UV spectrophotometry to determine the content of vitamin B4 tablets, which is simple and reliable.

$/></p>1 Instruments, reagents and drugs UV-1300PC UV spectrophotometer, vitamin B4 control, vitamin tablets, hydrochloric acid and other reagents are of analytical grade. 2 Experimental methods and results 2 1 Method Take vitamin reference substance, use 0.02mol / L hydrochloric acid solution to make a solution with a concentration of about 1ml, with 0.02mol / L hydrochloric acid solution as a blank, scan in the wavelength range of 200 ~ 30Onm, in Maximum absorption at 262 Â± 1 nm, consistent with standard specifications. Another take the amount of vitamin B4 tablets prescription amount of each auxiliary. The blank control solution was prepared with 0.02mol/L hydrochloric acid solution and scanned in the wavelength range of 200-300nm. The results showed that the excipient had only a small amount of absorption at the end, but no absorption near 262nm, and the wavelength of 262Â±1nm was used as the measurement wavelength. . 2.2 Linear relationship Accurately weigh about 20mg of vitamin B4 reference substance, put it in a lOOml volumetric flask, add 0.02mol/L hydrochloric acid solution to dissolve vitamin B4, add to the mark and shake. Accurately draw the above solutions 1, 2, 3, 4, 5ml, respectively, placed in a 50Inl volumetric flask. Dilute to the mark with 0.02mol/L hydrochloric acid solution, shake well, and take 0.02mol/L hydrochloric acid solution as blank, and obtain the regression equation: Y=l7.470 A-0.1845. r = 0.9999. The results showed that the concentration of vitamin B4 in the range of 4-20 Î¼g / ml showed a good linear relationship with the absorbance. 2.3 Recovery test Accurately weigh 105 Â° C dry constant weight of vitamins reference substance about 5Omg a total of 5 parts, placed in a 100mL volumetric flask, respectively, add a prescription amount of each auxiliary. Make a simulated vitamin B4 tablet, add 0.02mol / L hydrochloric acid solution, shake to make the vitamins dissolve, add 0.02mol / L hydrochloric acid solution to the mark, shake, filter, discard the primary filtrate, precision measurement Continue to filtrate 2ml, set in a lOOml volumetric flask, add O.02mol / L hydrochloric acid solution to the mark, shake well, determine the absorbance (A) at a wavelength of 262 Â± 1nm. The concentration was calculated from the regression equation and the recovery was calculated. 2.4 Stability test Take the above test solution and leave it at room temperature for 4 h. The measurement was performed once every 1 h, and the absorbance was substantially unchanged. 2.5 Determination of the sample Take 20 tablets of vitamin B4, accurately weighed, finely ground, accurately weighed the appropriate amount (about equivalent to vitamin B4 20mg), placed in a 100ml volumetric flask, and accurately weighed at l05 Â° C to constant weight About 50mg of vitamin B4 reference substance, placed in a lOOml volumetric flask, add 0.02mol / L hydrochloric acid solution to dissolve, according to the method of recovery test to determine the absorbance (A), according to the ratio of the two to calculate the equivalent of both vitamins The percentage of the indicated amount. At the same time, it is compared with the results measured by Kjeldahl method. 2.6 Repeated test Take the same batch of samples and take 5 samples under the â€œsample determinationâ€ to make the measurement. The average content was 94.3% and RSD=0.20% (n=5). Key words: ultraviolet spectrophotometry; vitamin B4; aesthetic instrument ; UV-1100</p> </div> </div> <div class="tech-detail-share"> <!- - Baidu Button BEGIN --> <div class="bdsharebuttonbox"> <a href="#" class="bds_qzone" data-cmd="qzone" title="Share to QQ Space"></a> <a href ="#" class="bds_tsina" data-cmd="tsina" title="Share to Sina Weibo"></a> <a href="#" class="bds_weixin" data-cmd="weixin" title ="Share to WeChat"></a> <span>Share to:</span> </div> <script>window._bd_share_config = { "common": { "bdSnsKey": {}, "bdText": " ", "bdMini": "1", "bdMiniList": false, "bdPic": "", "bdStyle": "2", "bdSize": "16" }, "share": {} }; with ( Document) 0[(getElementsByTagName($

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Determination of Vitamin B4 by UV-1100 Ultraviolet Spectrophotometry - Master's thesis - Dissertation

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